Lung Cancer Associated Fibroblasts
Increasing evidence in cancer literature indicates the importance of tumor associated fibroblasts. It is thought that these cells secrete cytokines and growth factors that may be necessary to potentiate tumor growth. CET's LCAF's are isolated from tumor tissue which has been enriched for fibroblast growth. Cells are then passaged to assure homogeneity. LCAF's can be grown to make conditioned media, which can then be tested by GC or HPLC for growth factors secreted, and also for co-culture with tumor cells of the lung.