Human Induced Pluripotetent Stem (iPS) Cell Characteristics
Human iPS cells are created by expressing modified Yamanaka iPS genes, (Oct-4, Sox-2, Klf-4 and C-Myc) in various target cells using CET’s proprietary gene delivery method and reprogramming and growth tissue culture media. iPS cells retain embryonic stem cell like characteristics and express Nanog, Oct-4, SSEA-4, TRA-1-60, alkaline phosphatase staining and teratoma formation. These cells are useful for tissue differentiation of all three germ layers, including the ectoderm, endoderm, and the mesoderm. Some examples of tissue differentiation applications include conversion into pancreatic islet cells, hepatocyte like cells, cardiomyocytes and neuron like cells
The following demonstrates a short video on how iPS cell differentiates into Cardiomyocyte. The movie clip shows spontaneous beating heart cells.