Footprint-Free iPS Cell Line (Human Multipotent Stem Cell)
This target cell is a rare and adherent cell found in human umbilical cord blood that has a lower level of MHC Class II expression. The iPS cell line created from these target cells is robust, pluripotent, and easy to manage using CET’s growth media. The iPS cell line was created using an episomal proprietary mix of vectors (containing Oct-4, Sox-2, Klf-4 along with p53 Anti-sense, EBNA-1) necessary to reprogram this target cell into induced pluripotent stem cells (iPSC). The cell line was validated for pluripotency based on colony morphology, alkaline phosphatase expression, and expression of SSEA-4. Cells are free of Mycoplasma. iPS colonies exhibit classical morphology and growth. Cell line is shipped on dry ice.
Media Usage Protocol (includes quality control flow cytometry data): Human Multipotent Stem Cell Induced Pluripotent Stem Cells Protocol.pdf