© 2019 Cellular Engineering Technologies.

Footprint-Free iPS Cell Line (Human Foreskin Fibroblast)

 

This iPS cell line was created using a normal human foreskin fibroblast. The iPS cell line was created using an episomal reprogramming containing a proprietary mix of vectors (containing Oct-4, Sox-2, Klf-4 along with p53 Anti-sense, EBNA-1) necessary to reprogram a newborn foreskin fibroblast into induced pluripotent stem cells (iPSC). The cell line was validated for pluripotency based on colony morphology, alkaline phosphatase expression, and expression of SSEA-4. Cells are free of Mycoplasma. iPS colonies exhibit classical morphology and growth. Cell line is shipped on dry ice. 

Media Usage Protocol (includes quality control flow cytometry data): Human Foreskin Fibroblast Induced Pluripotent Stem Cells Protocol.pdf